Tetracycline Antibiotics: Elucidating the Electrochemical Fingerprint and Oxidation Pathway

Herein, a complete study of the electrochemical behavior most commonly used tetracycline antibiotics (TCs) on unmodified carbon screen-printed electrodes (SPEs) is presented. In addition, oxidation pathway TCs SPE elucidated, for first time, with liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). Square wave voltammetry (SWV) was to fingerprint (EF) shaping different processes in pH range from 2 12. Their characteristic structure and subsequent EF offer possibility distinguishing this class other types. Under optimized parameters, calibration curves (TET), doxycycline (DOXY), oxytetracycline (OXY), chlortetracycline (CHL) Britton Robinson buffer solution (pH 9) exhibited linear between 5 100 µM excellent reproducibilities (RSDTET = 3.01%, RSDDOXY 3.29%, RSDOXY 9.78% RSDCHL 6.88% at 10 µM, N 3) limits detection (LOD) LODTET 4.15 LODDOXY 2.14 LODOXY 3.07 LODCHL µM. Furthermore, binary, tertiary, complex mixtures all were analyzed SWV investigate corresponding EF. A dual screening 4 9), together use custom-made Matlab script data treatment, allowed successful confirmation single presence unknown samples. Overall, work presents straightforward SPE, allowing future on-site identification residues real